ABSTRACT:
This paper concerns the development of an HPTLC fingerprint of rhizomes from Curcuma longa collected from geographically different parts of Chhattisgarh, India. The goal was to measure curcuminoids, the major bioactive components of turmeric, and determine the extent of variation within samples obtained from different locales. Samples were taken directly from rhizomes with ethanol and more polar solvents of increasing polarity via Soxhlet apparatus. Comparison was made on a standard curve of curcumin. Plates of silica gel 60F254 served as the stationary phase while using the mobile phase being chloroform:methanol v/v at the ratio 95:5. The samples were spotted using Camag Linomat V applicator and scanned densitometrically at 425 nm to detect curcuminoids such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). The mobile phase delivered sharp, symmetric peaks thus assuring quantity. The results indicated significant variation in curcuminoid content among the turmeric samples, thus implying a strong influence of geographical origin on the phytochemical composition of Curcuma longa. Such findings thus underpin the requirement for location-specific standardization for ensuring consistent therapeutic efficacy. Additionally, the study proves the efficiency of HPTLC as a robust, precise, and cost-effective method for curcuminoid profiling. The efforts of this research toward the standardization of turmeric quality, by linking variability in the phytochemicals to geographic sources, indicate its potential implications for therapeutic applications. The developed HPTLC fingerprint is a reliable tool for quality control, allowing the pharmaceutical and nutraceutical industries to ensure that efficacy and safety are achieved in their products.
Cite this article:
Jain A, Tiwary B, Bajaj S. Development of HPTLC Fingerprint of Curcuma longa Collected from Different Geographical Locations of Chhattisgarh for Quantification of Curcumin. Prob. Sci., 2024;1(2): 40–45.DOI: https://doi.org/10.5281/zenodo.14634757
[1].
Aderemi FA, Alabi OM. Turmeric
(Curcuma longa): an alternative to antibiotics in poultry nutrition.
Transl Anim Sci. 2023 Nov 30;7(1):txad133.
[2].
Zhang HA, Kitts DD. Turmeric
and its bioactive constituents trigger cell signaling mechanisms that protect
against diabetes and cardiovascular diseases. Mol Cell Biochem. 2021
Oct;476(10):3785-3814.
[3].
Fuloria S, Mehta J, Chandel A,
Sekar M, Rani NNIM, Begum MY, Subramaniyan V, Chidambaram K, Thangavelu L,
Nordin R, Wu YS, Sathasivam KV, Lum PT, Meenakshi DU, Kumarasamy V, Azad AK,
Fuloria NK. A Comprehensive Review on the Therapeutic Potential of Curcuma
longa Linn. in Relation to its Major Active Constituent Curcumin.
Front Pharmacol. 2022 Mar 25;13:820806.
[4].
Kotra VSR, Satyabanta L,
Goswami TK. A critical review of analytical methods for determination of
curcuminoids in turmeric. J Food Sci Technol. 2019 Dec;56(12):5153-5166.
[5].
Cobzac SCA, Olah NK, Casoni D.
Application of HPTLC Multiwavelength Imaging and Color Scale Fingerprinting
Approach Combined with Multivariate Chemometric Methods for Medicinal Plant
Clustering According to Their Species. Molecules. 2021 Nov 29;26(23):7225.
[6].
Tiwari D, Kewlani P, Gaira KS,
Bhatt ID, Sundriyal RC, Pande V. Predicting phytochemical diversity of
medicinal and aromatic plants (MAPs) across eco-climatic zones and elevation in
Uttarakhand using Generalized Additive Model. Sci Rep. 2023 Jul 5;13(1):10888.
[7].
Sharifi-Rad J, Rayess YE, Rizk
AA, Sadaka C, Zgheib R, Zam W, Sestito S, Rapposelli S, Neffe-Skocińska K,
Zielińska D, Salehi B, Setzer WN, Dosoky NS, Taheri Y, El Beyrouthy M,
Martorell M, Ostrander EA, Suleria HAR, Cho WC, Maroyi A, Martins N. Turmeric
and Its Major Compound Curcumin on Health: Bioactive Effects and Safety
Profiles for Food, Pharmaceutical, Biotechnological and Medicinal Applications.
Front Pharmacol. 2020 Sep 15;11:01021.
[8].
Bhuarya HK, Sastri ASRAS,
Chandrawanshi SK, Bobade P, Kaushik DK. Agro-Climatic Characterization for
Agro-Climatic Zone of Chhattisgarh. Int J Curr Microbiol Appl Sci.
2018;7(08):108–17.
[9].
Amalraj A, Pius A, Gopi S, Gopi
S. Biological activities of curcuminoids, other biomolecules from turmeric and
their derivatives - A review. J Tradit Complement Med. 2016 Jun
15;7(2):205-233.
[10].
Attimarad M, Ahmed KK,
Aldhubaib BE, Harsha S. High-performance thin layer chromatography: A powerful
analytical technique in pharmaceutical drug discovery. Pharm Methods. 2011
Apr;2(2):71-5.
[11].
Poliński S, Topka P, Tańska M,
Kowalska S, Czaplicki S, Szydłowska-Czerniak A. Effect of Grinding Process
Parameters and Storage Time on Extraction of Antioxidants from Ginger and
Nutmeg. Molecules. 2022 Oct 31;27(21):7395.
[12].
Badke S, Kakad K, Malode S.
High-performance thin-layer chromatography (hptlc) method development and
validation for determination of remogliflozin etabonate and vildagliptin in
bulk and its tablet formulation. Int J Appl Pharm. 2022 Jul 28;147–53.
[13].
Pothitirat W, Gritsanapan W.
Quantitative Analysis of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin
in the Crude Curcuminoid Extract from Curcuma longa in Thailand by TLC-
Densitometry. J Pharm Sci. 2005 Jan 1;32.
[14].
Kotra SR, Singh L, Goswami T. A
critical review of analytical methods for determination of curcuminoids in
turmeric. J Food Sci Technol. 2019 Aug 5;56.
[15].
Arya N, Prakash O, Verma AsK,
Vivekanand, Pant A. Variation in antioxidant potential of curcuma longa l.
collected from different ecological niches of western himalayan region. Int J
Pharm Pharm Sci. 2015 Jan 1;7:85–90.
[16].
Kushwaha P, Shukla B, Dwivedi
J, Saxena S. Validated high-performance thin-layer chromatographic analysis of
curcumin in the methanolic fraction of Curcuma longa L. rhizomes. Futur J Pharm
Sci [Internet]. 2021;7(1):178
[17].
Balekundri A, Mannur V. Quality
control of the traditional herbs and herbal products: a review. Futur J Pharm
Sci [Internet]. 2020;6(1):67. Available from:
https://doi.org/10.1186/s43094-020-00091-5.